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Image Search Results
Journal: Autophagy
Article Title: Kit-mediated autophagy suppression driven by a viral oncoprotein emerges as a crucial survival mechanism in Merkel cell carcinoma
doi: 10.1080/15548627.2025.2477385
Figure Lengend Snippet: MCPyV truncated LT induces paranuclear retention and stabilization of KIT. (A) Immunofluorescence detection of KIT (green) and MCPyV LT (CM2B4, red) or sT (CM8E6, red) in KIT-HEK293 cells transfected with MCPyV T-antigens or vector control ( n = 6). (B) Top : illustration of the expression constructs of LT339 and the VPS39-interaction defective mutant LT339 W209A . Bottom : Representative images showing the effect of LT339 and LT339 W209A mutant on localization of KIT (green). LT was detected by CM2B4 (red). ( n = 3) (C) Immunoblots showing the effect of the LT339 and LT339 W209A on KIT expression. The quantification of KIT level is shown below the immunoblots ( n = 9). (D) Top : immunoblot analysis of the effect LT339 and LT339 W209A on KIT protein stability in the presence of cycloheximide (CHX) up to 4 h. Bottom : quantification of KIT protein stability after normalization to 0 h time point ( n = 4). (E) Immunoblots showing KIT protein stability in KIT-HEK293 cells transfected with LT339, LT339 W209A or plasmid control (CTR) treated with KIT ligand (KITLG, 100 ng/mL) or solvent control (PBS) in the presence of CHX (100 μg/mL). (F) Quantification of KIT protein stability after normalization to 0 h time point ( n = 5). Solid lines represent PBS control (KITLG − ) and dotted lines represent KITLG treatment (KITLG + ). (A and B) Nuclei were stained by DAPI (blue). Scale bar: 10 μm. Numbers below the images refer to the proportion of cells with KIT paranuclear dot-like staining to the total number of cells analyzed. (C, D and F) Error bars represent mean ± SEM. * p < 0.05, *** p < 0.001, ns = not significant were calculated by one-way ANOVA with post-hoc Tukey’s test (C), two-way ANOVA (D) or two-way ANOVA with post-hoc Bonferroni’s test (F).
Article Snippet: For KITLG induced KIT degradation experiments, KIT-293 cells were transfected with LT339, LT339 W209A or CTR for 48 h, followed by addition of CHX (100 μg/mL) and
Techniques: Immunofluorescence, Transfection, Plasmid Preparation, Control, Expressing, Construct, Mutagenesis, Western Blot, Solvent, Staining
Journal: Cell Journal (Yakhteh)
Article Title: The Effect of Baicalin as A PPAR Activator on Erythroid Differentiation of CD133 + Hematopoietic Stem Cells in Umbilical Cord Blood
doi:
Figure Lengend Snippet: Expressions of GPA (A) and (B) CD71 surface markers evaluated by flow cytometry at day 7 of erythroid differentiation in IMDM-containing cytokines, SCF (20 ng/ml) and EPO (4.5 U/ml) in the presence of baicalin at concentrations of 20 and 50 μM and control isotypic antibody. GPA; Glycophorin A, CD; Cluster of differentiation, IMDM; Iscove’s modified dulbecco medium, SCF; Stem cell factor, EPO; Erythropoietin, M; Monocolonal, IgG; Imonoglubolin, FITC; Fluorescein isothiocyanate, TfR; Transferrin receptor and PE; Phycoerythrin.
Article Snippet: The medium was contained recombinant
Techniques: Flow Cytometry, Control, Modification
Journal: Cell Journal (Yakhteh)
Article Title: The Effect of Baicalin as A PPAR Activator on Erythroid Differentiation of CD133 + Hematopoietic Stem Cells in Umbilical Cord Blood
doi:
Figure Lengend Snippet: Expressions of GPA (A) and CD71 (B) surface markers evaluated by flow cytometry at day 7 of erythroid differentiation in IMDM that contained SCF (20 ng/ml) and EPO (4.5 U/ml) in the presence of troglitazone at concentrations of 0.1 and 10 μM and control isotypic antibody. GPA; Glycophorin A, CD; Cluster of differentiation, IMDM; Iscove’s modified dulbecco medium, SCF; Stem cell factor, EPO; Erythropoietin, M; Monocolonal, IgG; Imonoglubolin G, FITC; Fluorescein isothiocyanate, TfR; Transferrin receptor and PE; Phycoerythrin.
Article Snippet: The medium was contained recombinant
Techniques: Flow Cytometry, Control, Modification